Single-cell Analysis / R package
DropletUtils
Read common droplet data formats and distinguish candidate cells from background droplets.
Best fit
Bioconductor users checking unfiltered droplet RNA expression data.
What you need
R and Bioconductor; cell-calling methods need counts that still contain background barcodes.
What to check
A prefiltered matrix cannot recover discarded droplets, and low-RNA cells can be missed.