Bioimage Analysis / Fiji plugin
BigStitcher
Register and fuse tiled or multiview microscopy acquisitions to build a larger volume.
Best fit
Light-sheet and fluorescence imaging teams assembling a volume from overlapping acquisitions.
What you need
Fiji with BigStitcher, original tiles or views, and enough storage for the fused output.
What to check
Poor overlap, sample drift, or mismatched calibration can leave seams or misalignment. Check pairwise alignment before fusion.